. 2013 Apr 18;9(4):e1003267. doi: 10.1371/journal.ppat.1003267

Table 2. Plasmids used in this study.

Designation	Relevant Characteristics	Reference/source
pBS34	pBluescript II KS+ (Carb^R, Kan^R, KSAC cassette)	[10]
pCR2.1	Cloning vector; Carb^R, Kan^R	Invitrogen
pEP185.2	oriR6K mobRP4 cat (Cm^R)	[50]
pFR2	‘aer’ gene fragment cloned into pGP704; insertion of pFR2 into the IR715 chromosome aer gene.	This Study
pFR3	‘tsr’ gene fragment cloned into pEP185.2; insertion of pFR3 into the IR715 chromosome disrupts tsr.	This Study
pFR4	‘aer’ gene fragment cloned into pEP185.2; insertion of pFR4 into the IR715 chromosome disrupts aer.	This Study
pFR5	aer open reading frame and promoter cloned into pWSK29	This Study
pFR6	tsr open reading frame and promoter cloned into pWSK29	This Study
pKD3	oriR6K, Cm^R	[42]
pKD4	oriR6K, Kan^R	[42]
pKD46	Plasmid encoding lambda red recombinase	[42]
pSPN57	pRDH10(Cm^R, ΔinvA(−9 to +2057))	[10]
pSPN60	pSPN57(Cm^R, Kan^R, ΔinvA::KSAC)	This Study
pWSK29	Cloning vector; Carb^R	[51]
pWSK129	Cloning vector; Kan^R	[51]