Figure 7. Serum levels of soluble mediators and neutrophil activation in DENV-infected mice treated once with 7.5% NaCl at day 6 post-infection.

Groups of 8–9 week-old AG129 mice were left uninfected or were sc infected with D2Y98P-PP1 virus and at day 6 pi., they were treated with a single bolus (iv; 4 mL/kg body weight) of 7.5% NaCl (HTS) or PBS as indicated. (A) At the indicated time points post-infection, 5 mice per group were euthanized and bled for measurement of IL-6, TNF-α, complement protein C5a, MMP9, VEGFA and its receptor VEGFR2. Results are expressed as the mean ± SD of five individual samples per time point. (B) At 24 hrs post-HTS or PBS treatment, 5 mice per group were bled and FACS analysis was performed to detect surface expression of CD62L and CD11b, and intracellular production of TNF-α and VEGFA.