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. 2013 Apr 18;9(4):e1003473. doi: 10.1371/journal.pgen.1003473

Figure 5. Smt3 is necessary for the expression of Dib and Ftz-f1 in follicle cells.

Figure 5

(A–G) Single plane confocal micrographs were taken under the same intensity settings in smt3 knockdown and control ovaries. Nuclei are labelled with DAPI (purple). F-actin cytoskeleton is shown in blue in B, D, F and G. GFP is shown in blue in C and E. Follicle cells are indicated by yellow dotted lines in B–G. (A′–G′) Single green channels are shown in black and white. Close-up of the follicle cells are shown in insets in D′–G′. Scale bars indicate 20 µm. (A) Expression of Smt3 (green) in the Drosophila germarium and egg chambers. Arrowhead points to follicle cells (FC). (B–C) Smt3 (green) expression levels in follicle cells in T155-Gal4>UAS-GFP;UAS-smt3i (Smt3i) (C) is severely reduced compared with the control (B). (D–G) Stage 8 and early stage 9 egg chambers showing in green the expression of Dib (D, E) or Ftz-f1 (F, G) in T155-Gal4>UAS-GFP;UAS-smt3i or T155-Gal4>UAS-smt3i (E, G) compared with the controls (D, F).