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. 2013 Apr 18;8(4):e60888. doi: 10.1371/journal.pone.0060888

Figure 5. VSMC differentiation marker genes were downregulated in Drosha cKO mice.

Figure 5

A. Sections of thoracic aorta of control and Drosha cKO at E13.5 were stained with αSMA antibody. B. The expressions of αSMA, SM22, CNN1, and GAPDH in umbilical arteries of control and cKO mice at E13.5 were detected by Western blot. Data represent mean ± SD (n = 5). C. VSMC marker genes in KO and control VSMCs generated using Ade-con and Ade-Cre viruses were detected by Western blot. Three separate experiments were performed. D. VSMC marker genes in KD and control VSMCs were examined using Western blot. Protein bands were quantified by densitometry for analysis of significant differences (*P<0.05,**p<0.01, ***P<0.001).