Figure 2. Identification of the CIP2A proximal promoter region. (A) and (D) Diagrammatic representation of the full length and sequentially deleted CIP2A promoter constructs used in this study. The transcription start site (TSS) is numbered as +1 and the constructs are numbered with reference to the TSS from 5′–3′ ends. (B) Human cervical carcinoma cells (HeLa), (C) liver hepatobalstoma cells (HepG2), and (E) endometrial carcinoma cells (ECC-1) were transfected with CIP2A promoter constructs shown in Figure 2A or 2D and assayed for luciferase activity after 48 h as described in the Materials and Methods. Fold increase in relative luciferase activity (RLA) was compared with pGL4-basic (set as 1). Normalization in transfection efficiency was performed by co-transfection with pRL-TK (Renilla expression vector). The mean ± SD are from three different experiments, each performed in triplicate.