Figure 8. Ectopic expression of ETS1 and ELK1 together rescues CIP2A expression from 3′ UTR siRNA treatment. HeLa cells were transiently transfected with 100 nM of ETS1 and ELK1 3′ UTR siRNA together or non-targeting (NT) siRNA as the negative control. ETS1 and ELK1 cDNA were cloned into pCDN4-His Max Topo expression vector (Invitrogen, K864–20) utilizing the primers mentioned in Table 4, generating Ets1-Topo and Elk1-Topo. Sequences of the clones were verified and 1 µg was utilized for ectopic expression in HeLa cells following 3′ UTR siRNA treatment. Cells transfected with the empty vector served as a negative control. (A) western blot analysis of CIP2A, Ets1, Elk1 and GAPDH protein expression levels was performed 72 h after transfection and (B) qRT-PCR was conducted at 48 h after transfection to confirm rescue of CIP2A mRNA. **p < 0.01.