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. 2013 Apr 18;9(4):e1003323. doi: 10.1371/journal.ppat.1003323

Figure 5. Antigenic properties of FH-binding HVRs.

Figure 5

(A) Isolated HVRs show limited or no antigenic cross-reactivity. Antiserum to one HVR (specified at the top of a panel) was analyzed for reactivity with four immobilized HVRs, as indicated. The HVRs of M5 and M18 exhibited limited cross-reactivity, while the HVR of M6 did not cross-react with the other two HVRs. The non-FH-binding M1-HVR was included as a control. Pre-immune sera were used to obtain background values, which were subtracted from the values obtained with immune sera. (B) Binding of FH to the HVR of an M protein is inhibited by antiserum to the corresponding HVR. The biotinylated M protein indicated above each panel was used to detect FH immobilized in microtiter wells. The binding was inhibited with rabbit anti-HVR serum, diluted as indicated. Preimmune serum was used as a control.