Skip to main content
. 2013 Apr 18;9(4):e1003458. doi: 10.1371/journal.pgen.1003458

Figure 3. Effects of point mutations in the DUS on quantitative transformation and DNA binding and uptake of N. meningitidis MC58.

Figure 3

(A) Quantitative transformation of Neisseria meningitidis strain MC58 with plasmid DNA containing a panel of modified DUS sequences. Transformation rates are shown as percentage relative to the AT-DUS. Standard deviations from 7 independent experiments are indicated by bars. (B) DNA binding (gray bars) and uptake (white bars) assay with radiolabelled plasmid DNA. Average values from 4 independent experiments are shown as percentage of total DNA added. T-test results for (A) transformation rates compared to AT-DUS and (B) for DNA binding versus uptake are indicated by stars (p≤0.2 = *, p≤0.05 = **, p≤0.001 = ***). DUS sequence transversions are given as abscissa labels. The sequence logo is based on the 2742 occurrences of DUS, with a single nucleotide divergence allowed, found in the genome of N. meningitidis MC58.