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. 2013 Apr 18;9(4):e1003458. doi: 10.1371/journal.pgen.1003458

Table 1. DUS–dependent transformation frequencies.

Strain DNA DUS Frequency Ratio1
Neisseria meningitidis MC58 pOHA-D4 AT-DUS 4,4E-06 (SD = 4,1E-06) 1
pSAF-DV-48 AG-DUS 3,9E-06 (SD = 4,3E-06) 0,90
pSAF-DV-49 AG-mucDUS 1,6E-06 (SD = 1,3E-06) 0,36
pSAF-DV-50 AG-kingDUS 1,0E-09 (SD = 1,8E-09) 9 CFU in total3 0,0002
pSAF-DV-51 AG-eikDUS 02 n.a.4
pSAF-DV-52 reverse USS 02 n.a.4
pSAF-DV-53 USS 2 CFU in total3 n.a.4
Neisseria elongata subsp. glycolytica 6171/75 rpsL - strepR AG-DUS 3,1E-07 (SD = 2,4E-07) 1,39
rpsL - strepR AT-DUS 2,2E-07 (SD = 1,2E-07) 1
rpsL - strepR AG-mucDUS 9,2E-09 (SD = 1,5E-08) 0,04
Kingella denitrificans ATCC 33394 rpsL - strepR AA-king3DUS 9,2E-06 (SD = 6,7E-06) 90
rpsL - strepR AT-DUS 1,0E-07 (SD = 6,8E-08) 1
Eikenella corrodens 31745 rpsL - strepR AG-eikDUS 6,4E-05 (SD = 3,5E-05) n.a.4
rpsL - strepR AT-DUS 02 n.a.4

Frequencies of transformation in selected bacterial species with different DUS dialects displaying cross-species DUS transformation ratios. Experiments were performed at least three times.

1 transformation frequency relative to the AT-DUS transformation frequency in the same species.

2 erythromycin-resistant transformants could not be detected in any of all experiments.

3 cumulative number of CFU in all performed experiments.

4 not applicable.

SD = standard deviation.