Table 1. DUS–dependent transformation frequencies.
Strain | DNA | DUS | Frequency | Ratio1 |
Neisseria meningitidis MC58 | pOHA-D4 | AT-DUS | 4,4E-06 (SD = 4,1E-06) | 1 |
pSAF-DV-48 | AG-DUS | 3,9E-06 (SD = 4,3E-06) | 0,90 | |
pSAF-DV-49 | AG-mucDUS | 1,6E-06 (SD = 1,3E-06) | 0,36 | |
pSAF-DV-50 | AG-kingDUS | 1,0E-09 (SD = 1,8E-09) 9 CFU in total3 | 0,0002 | |
pSAF-DV-51 | AG-eikDUS | 02 | n.a.4 | |
pSAF-DV-52 | reverse USS | 02 | n.a.4 | |
pSAF-DV-53 | USS | 2 CFU in total3 | n.a.4 | |
Neisseria elongata subsp. glycolytica 6171/75 | rpsL - strepR | AG-DUS | 3,1E-07 (SD = 2,4E-07) | 1,39 |
rpsL - strepR | AT-DUS | 2,2E-07 (SD = 1,2E-07) | 1 | |
rpsL - strepR | AG-mucDUS | 9,2E-09 (SD = 1,5E-08) | 0,04 | |
Kingella denitrificans ATCC 33394 | rpsL - strepR | AA-king3DUS | 9,2E-06 (SD = 6,7E-06) | 90 |
rpsL - strepR | AT-DUS | 1,0E-07 (SD = 6,8E-08) | 1 | |
Eikenella corrodens 31745 | rpsL - strepR | AG-eikDUS | 6,4E-05 (SD = 3,5E-05) | n.a.4 |
rpsL - strepR | AT-DUS | 02 | n.a.4 |
Frequencies of transformation in selected bacterial species with different DUS dialects displaying cross-species DUS transformation ratios. Experiments were performed at least three times.
1 transformation frequency relative to the AT-DUS transformation frequency in the same species.
2 erythromycin-resistant transformants could not be detected in any of all experiments.
3 cumulative number of CFU in all performed experiments.
4 not applicable.
SD = standard deviation.