Figure 2. Graphical User Interface of TrichEratops.

TrichEratops offers 4 different views of the leaf surface. The 2D sharpened image view (A) is used to mark trichome positions and their classes (red, purple, blue and green dots). Additionally, the leaf-centric xy-coordinate system can be set (blue axes), and the calibration marks for z-stacking can be specified (white and dark blue dot on the top half of the image). The 3D surface view (B) serves as a visual control of the reconstruction quality. The shortest paths between trichomes can be included optionally. The top control panel offers functionality for loading, saving, processing, and analysis of the image stacks.