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. 2013 Apr 17;8(4):e61013. doi: 10.1371/journal.pone.0061013

Figure 2. PQQ suppresses the mRNA expression of c-Fos, NFATc1, and TRAP in BMMs treated with RANKL.

Figure 2

(A–C) BMMs were pretreated with or without PQQ (15 µM) for 1 h and with RANKL (100 ng/ml) for the indicated periods. The mRNA expression of the indicated genes was analyzed by real time RT-PCR. Data are presented as mean ± SD; 0: Blank Control; #p<0.05 vs. Blank Control; *p<0.05 vs. DMSO+RANKL. (D) PQQ inhibits the expression of c-Fos and NFATc1 induced by RANKL. BMMs were pretreated with or without PQQ (15 µM) for 1 h and were treated with RANKL (100 ng/ml) for the indicated periods. Cells were lysed in the lysis buffer, and lysates were analyzed by Western blotting with antibodies against c-Fos, NFATc1, and actin. The intensities of the protein bands were analyzed and normalized to actin. Similar results were obtained in at least 3 independent experiments.