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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Cancer Res. 2013 Feb 27;73(8):2671–2681. doi: 10.1158/0008-5472.CAN-12-3513

Fig. 3.

Fig. 3

miR-290-3p targets the 3′UTR of Arid4b. Arid4b expression was measured by (a) qRT-PCR and (b) Western blot in all four stable cell lines with upregulated miR-290. c) Luciferase activity was measured after transfection of a vector containing the 3′-UTR of ARID4B adjacent to a Renilla gene with miR-290-3p or miR-290-5p. d) Two miR-290-3p targeted regions within the 3′-UTR of ARID4B were identified and then mutated using 2 point mutations each. e) Luciferase activity was measured for the WT 3′UTR of ARID4B and the mutants after miR-290-3p transfection. *p < 0.05, **p < 0.01, ***p < 0.001.