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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Cancer Res. 2013 Feb 25;73(8):2551–2562. doi: 10.1158/0008-5472.CAN-12-4108

Fig 4. Biological affects of FGF19 knockdown on PCa cells.

Fig 4

A, B. FGF19 was transiently knocked down using two different shRNAs and mRNA levels evaluated by Q-RT-PCR. Proliferation and invasion were evaluated on parallel plates as described in Materials and Methods. A: DU145 cells; B: PC3 cells. C. FGF19 was stably knocked down using two different shRNAs. Protein expression was determined using an FGF19 ELISA. Proliferation and invasion were evaluated on parallel plates as described in Materials and Methods. For A–C, experimental data is expressed relative to vector control cells (100%). The mean +/− standard deviation is shown. D. PC3 cells with knockdown of FGF19 (FGF19 sh1, n=18) or vector controls (n=14) were inoculated subcutaneously on day 1. Calculated tumor volume is shown at intervals from day 13 to day 32. Mean +/− SEM is shown. Statistically significant differences from controls are indicated by asterisks for all experiments.