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Influence of covalent kinetic stabilizers 5, 9, (3.6 μM) on the rate of WT-TTR tetramer dissociation relative to WT-TTR (1.8 μM tetramer) in the absence of a covalent kinetic stabilizer. Dissociation and unfolding of TTR in 6 M urea is monitored by far-UV circular dichroism at 215 – 218 nM over 144 h after a 1 h preincubation period with covalent kinetic stabilizer.
