Figure 3.

Invasion analysis and enzyme activity of HTR-8/SVneo cells affected by NET expression. (A) The pcDNA-NET concentration-dependent invasion activity of HTR-8/SVneo cells. (B) The siNET concentration-dependent invasion activity of HTR-8/SVneo cells. (C) The enzyme activity of MMP-2 (left) and MMP-9 (right) in conditioned medium due to pcDNA-NET modulation. (D) The enzyme activity of MMP-2 (left) and MMP-9 (right) in conditioned medium due to siRNA-NET modulation. The columns in the graph represent the intensity of MMP-2 and MMP-9 measured in triplicate. Significant difference are indicated by ^ap<0.05 vs. Mock. The values are expressed as the means±SE of three separate experiments. NET, norepinephrine transporter; M, mock control; MMPs, matrix metalloproteinase.