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(A) Sperm chromatin structure assessed by toluidine blue staining. Sperm cell heads with good chromatin structure were light blue; those of abnormal chromatin structure were deep violet (arrow, ×1,000). (B) Sperm chromatin condensation assessed by aniline blue. Immature sperm stained dark blue by the eosin counterstain (arrowheads, ×1,000).
