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. Author manuscript; available in PMC: 2013 Apr 19.
Published in final edited form as: Methods Mol Biol. 2013;936:313–324. doi: 10.1007/978-1-62703-083-0_24
Components Megaplex RT reaction volume (μL)
Megaplex RT primer mix A or B (10×) 0.75
dNTPs with dTTP (100 mM total) 0.15
MultiScribe Reverse Transcriptase (50 U/(μL) 1.50
RT Buffer (10×) 0.75
MgCl2 (25 mM) 0.90
RNase Inhibitor (20 U/μL) 0.09
Nuclease-free water 0.36
Total 4.5