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. Author manuscript; available in PMC: 2013 Apr 19.
Published in final edited form as: Methods Mol Biol. 2013;936:313–324. doi: 10.1007/978-1-62703-083-0_24
Components Megaplex Pre-amplification reaction volume (μL)

Megaplex Preamp Primer Mix A or B(10×) 4.0

Taqman® Preamp Master Mix (2×) 20.0

Nuclease-free water 8.5

Total 32.5