| Components | RT-PCR reaction volume (μL) |
|
| |
| Pre-Amplification product (no dilution) | 9.0 |
|
| |
| Taqman® Master Mix, no UNG (2×) | 450.0 |
|
| |
| Nuclease-free water | 441.0 |
|
| |
| Total | 900.0 |
|
| |
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