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. 2013 Mar 19;32(8):1141–1154. doi: 10.1038/emboj.2013.56

Figure 7.

Figure 7

Depletion of CEP135 impairs the recruitment of CPAP to the centriole and inhibits CPAP-induced centriole elongation. (A) CPAP-myc-inducible cells were transfected with siControl or siCEP135 for 2 days, followed by tetracycline induction for another 2 days and analysis by immunofluorescent confocal microscopy using the indicated antibodies. (B) Quantitative analysis of the relative centriolar intensity of CPAP-myc in siRNA-treated cells. (C) Quantitative analysis of the elongated centrioles induced by CPAP-myc overexpression in siControl- and siCEP135-treated cells. Bar values are means±s.d of three independent experiments; P=0.0011. (D) Histogram illustrating the percentages of cells showing elongated centrioles (counted by Ac-tubulin; n=100 in triplicate). In siRNA rescue experiments (D–F), CPAP-myc-inducible cells were transfected with siCEP135, followed by transfection of a vector encoding siRNA-resistant EGFP-CEP135-R or EGFP, tetracycline induction as shown in (H). The cells were then analysed by IB (E) and immunofluorescent staining (F) using the indicated antibodies. Elongated centrioles were effectively rescued by the expression of EGFP-CEP135 in siCEP135-treated cells (D, F). (G) CEP135 depletion inhibits CPAP-induced centriole elongation.