Figure 1.

CC_elastase purification. (A) Schematic representation of the regions of TraN/VirB7, TraO/VirB9 and TraF/VirB10 present in the CC_elastase complex. Domains corresponding to the VirB9-binding domain (B9BD), the signal peptide (SP), the N-terminal TM helix and the C-terminal domains (CTD) are shown in darker colours. (B) SDS–PAGE of the CC_elastase and the FLCC complexes. For the CC_elastase complex, the first aa in the bands identified using Edman degradation are indicated. The CC_elastase complex is composed of three major bands: full-length TraN/VirB7 and TraO/VirB9 and cleaved TraF/VirB10. In addition two minor byproducts are observed, both consisting of cleaved TraO/VirB9. Molecular weight standards (Std) are indicated. (C) Size exclusion chromatography of the CC_elastase complex using a Superose 6 10/300 (GE Healthcare). The mobility of the pKM101 FLCC and the OL complex is also indicated by blue arrows in the size exclusion chromatography plot.