Figure 1.

Auxin-induced phosphorylation of AtS6K1 at TOR-specific residue T449 regulates interaction with eIF3. (A) eIF3c or TOR immunoprecipitated from WT extracts was assayed for complex formation between eIF3c, TOR, S6K1, raptor, and ARF-GTPase. Input, 5% of immunoprecipitation (IP) or normal rabbit serum (RS). (B) Suspension-culture cells treated with NAA or Torin-1 for 0, 4, and 6 h, lysed assayed by immunoblot analysis. (C) eIF3c immunoprecipitated from extracts in (B) and assayed for association with TOR and S6K1 by immunoblotting. Source data for this figure is available on the online supplementary information page.

Source Data for Figure 1A ^{(409.7KB, pdf)}

Source Data for Figure 1C ^{(4.5MB, pdf)}