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. 2013 Mar 22;32(8):1103–1114. doi: 10.1038/emboj.2013.62

Figure 6.

Figure 6

Downregulation of OTUB1 diminishes TWEAK signalling. (A) A2058 cells were transfected with control or OTUB1-specific siRNA oligos and 48 h later treated with TWEAK (100 ng/ml) for indicated time periods. Cellular lysates were examined by western blotting with indicated antibodies. (B) HT1080 cells were transfected with control or OTUB1-specific siRNA oligos and 48 h later treated with TWEAK (100 ng/ml), BV6 (2.5 μM), or TNFα (20 ng/ml) for indicated time periods. Supernatant from cells was examined for production of IL-8 cytokine by Luminex Technology.