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HTB-186 MB cell line susceptibility to: resting NK cells, IL-15 stimulated NK cells, NKG2D, MICA, ULBP-2 and HLA class I blocking experiments at different effector target (E/T) ratio. Blocking MICA, ULBP-2 on HTB-186 cells and NKG2D on NK cells reduced NK cell mediated cytotoxicity. HLA class I blocking on HTB-186 cells and IL-15 stimulated NK cells increased NK cell mediated cytotoxicity.
