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. Author manuscript; available in PMC: 2013 Apr 19.
Published in final edited form as: Adv Exp Med Biol. 2008;613:369–375. doi: 10.1007/978-0-387-74904-4_43

Figure 2.

Figure 2

Retinal adhesion is dramatically reduced in β5 knockout mice. (a) After enucleation and lens/cornea removal, retinas are swiftly peeled off opened eyecups creating shearing forces to assess retinal adhesion. In retinas with normal retinal adhesion, apical cellular domains of RPE largely remain attached to the outer surface of the neural retina. (b) Whole-mount of peeled retina, shown outer retina up, demonstrating that β5 knockout retina retains significantly less RPE pigment than wild-type retina. (c) Quantification of RPE pigment in retina peeled at different times of day. β5 knockout retina shows reduced pigment contents and attenuated adhesiveness peak compared to wild-type retina. (d) Representative immunoblots of individual peeled retina confirming the melanin pigment results. Modified from Finnemann and Chang and from Nandrot et al. (2006) with permission from Humana Press Inc. and the American Physiological Society.