Figure 1.

MiR-195 and miR-497 are downregulated in colon cancer cells. (a) Total RNA from five representative paired CRC tissues and adjacent normal mucosa were subjected to miR array analysis. The filtered miR array data were subjected to unsupervised hierarchical clustering analysis. The metric was set as the ‘euclidean distance’. (b) Quantitative analysis of miR array data showing downregulation of miR-195 and miR-497 and upregulation of miR-424 in CRC tissues compared with paired adjacent normal mucosa. The data shown are average fold changes of individual miR expression in CRC tissues relative to normal mucosa. (c) qRT–PCR analysis of miR-195 (upper), miR-497 (middle) and miR-424 (lower) in total RNA from CRC tissues and paired adjacent normal colon mucosa showing that miR-195 and miR-497 are downregulated, whereas miR-424 are upregulated in the majority of colon cancers. The data shown are average fold changes (the mean±s.e. of three individual experiments) of individual miR expression CRC tissues relative to normal mucosa. (d) Regression analysis showing that the levels (folds changes in CRC tissues relative to normal mucosa) of miR-195 and miR-497 are significantly correlated in CRC tissues (R²=0.891, P<0.01). (e) qRT–PCR analysis of miR-195 (upper) and miR-497 (lower) in total RNA from indicated colon cancer cell lines and the normal colon epithelial line CRL-1831. The data shown are average fold changes (the mean±s.e. of three individual experiments) of individual miR expression in each colon cancer line relative to CRL-1831 cells.