Fig. 1.
E23A inhibits complement activity both in vitro and in vivo. (A) Human O serum was incubated with increasing concentrations of E23A in DMSO and then added to human AB erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM. (B) NHS was incubated with increasing concentrations of E23A in DMSO and then added to mannan coated plates. C3 deposition was measured by ELISA. (C) Three male Wistar rats received IP injection of either 20 mg E23A or vehicle control (DMSO). Blood was collected before and one hour after injection, from which serum was isolated and tested in hemolytic assays with erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM.