Fig. 4.

Polar assortant (PA) inhibits classical pathway hemolysis and binds C1q CLR. (A) Hemolytic assays performed with different concentrations of PA in Factor B-depleted serum. Factor B-depleted serum was incubated with 0.77 mM of peptide inhibitors in DMSO and then added to sensitized erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM. (B) C1q, GHR, CLR or gelatin (negative control) were absorbed to a microtiter plate and incubated with various amounts of biotinylated PA which was then detected with a streptavidin HRP conjugate. Data are the means from 3 independent experiments. Error bars denote SEM.