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Effect of PA deletions on hemolytic activity. (A) PA sequences with N- and C-terminal deletions. (B) Hemolytic assays performed with PA synthesized by different vendors (GenScript (GS) or NEP), and PA peptides with amino and carboxy terminus deletions in Factor B-depleted serum. Factor B-depleted serum was incubated with 0.77 mM of peptide inhibitors in DMSO and then added to sensitized erythrocytes. Values are the means of three independent experiments. Error bars represent the SEM.
