Table 1.
Immobilized Protein | Soluble analyte | kon (×104) M−1s−1 | koff (×10−4) s−1 | KDa (nM) |
---|---|---|---|---|
E23A | C1q | 11.1 ± 2.34 | 6.52 ± 0.351 | 6.08 ± 0.966 |
Δ8-22 | C1q | 19.8 ± 0.395 | 10.6 ± 3.94 | 5.43 ± 2.10 |
PA | C1q | 2.58 ± 0.596 | 8.08 ± 0.206 | 33.3 ± 8.49 |
KD values were calculated from kon/koff for each experiment and were averaged from at least two separate experiments.