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. 2013 Feb 27;28(3):341–350. doi: 10.1093/mutage/get010

Fig. 2.

Fig. 2.

Analysis of gene disruptions genes in newly created strains by PCR. For primers used, see Figure 1. (A) ogt and cat genes: lanes 1 and 7, wild-type (NR10382); lanes 2 and 8, ogt (KT233); lanes 3 and 9, mutS (NR12896); lanes 4 and 10, mutS ada ogt (KT11141); lanes 5 and 11, mutL ada ogt (KT21181); lanes 6 and 12, mutH ada ogt (KT31131), M1, 100-bp marker; M2, 1-kb marker. (B) ada and tetA genes: lanes 1 and 7, wild-type (NR10382); lanes 2 and 8, ogt (KT233); lanes 3 and 9, mutS (NR12896); lanes 4 and 10, mutS ada ogt (KT11141); lanes 5 and 11, mutL ada ogt (KT21181); lanes 6 and 12, mutH ada ogt (KT31131); M, 1-kb marker. (C) uvrA and tetA genes: lanes 1 and 7, wild-type (NR10382); lanes 2 and 8, ogt (KT233); lanes 3 and 9, mutS (NR12896); lanes 4 and 10, mutS ada ogt (KT11141); lanes 5 and 11; mutL ada ogt (KT21181); lanes 6 and 12: mutH ada ogt (KT31131); M, 1-kb marker.