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. 2013 Feb 27;28(3):341–350. doi: 10.1093/mutage/get010

Fig. 7.

Fig. 7.

Complementation of MMR deficiencies by plasmid-carried wild-type mutS, mutL and mutH genes. (A) MMS mutagenesis. Strains used are 1 (grey bar) KT01121 (ada ogt); 2 (white bar) KT11141 (mutS ada ogt); 3 (black bar) KT11141 (mutS ada ogt) with pMQ315 (mutS +); and 4 (dark grey bar) KT11141 (mutS ada ogt) with pBR322. (B) EMS mutagenesis. Strains are 5 (grey bar) NR12999 (uvrA); 6 (white bar) KT10021U (mutS uvrA); 7 (black bar) KT10021U (mutS uvrA) with pMQ315 (mutS +); 8 (dark grey bar) KT10021U (mutS uvrA) with pBR322. (C) MNNG mutagenesis. Strains are 1 (grey bar) KT01121 (ada ogt); 2 (white bar) KT21181 (mutL ada ogt); 3 (black bar) KT21181 (mutL ada ogt) with pMQ350 (mutL+); 4 (dark grey bar) KT21181 (mutL ada ogt) with pBR322; 5 (white bar) KT31131 (mutH ada ogt); 6 (black bar) KT31131 (mutH ada ogt) with pRH71-17 (mutH+); 7 (dark grey bar) KT31131 (mutH ada ogt) with pBR322. Statistical analysis was performed using the Student’s t test. **P > 0.01 and *P > 0.05 compared with the mutant frequency for each parental strain with MMR deficiency.