Table II.
Alkylation-induced mutability of repair-deficient E. coli strainsa
| Genotype | MMS (5mM) | MNNG (0.005mM) | MNU (5mM) | EMS (50mM) | ENNG (0.5mM) | ENU (25mM) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ratio vs. wild-type (MF × 107) | Survivalb (%) | 1 (7.4±1.0) | 91 | 1 (3.4±0.9) | 86 | 1 (11.9±3.8) | 81 | 1 (117.5±4.7) | 83 | 1 (226.2±55.3) | 64 | 1 (81.6±6.8) | 86 |
| ada, ogt | 173.3 | 68 | 433.9 | 87 | 148.2 | 95 | 2.5 | 86 | 3.1 | 72 | 2.1 | 79 | |
| uvrA | 1.2 | 88 | 0.3 | 101 | 7.5 | 48 | 9.6 | 96 | 2.8 | 55 | 17.5 | 78 | |
| mutS | 5.8 | 87 | 3.3 | 97 | 3.2 | 77 | 1.7 | 81 | 1.2 | 63 | 0.8 | 97 | |
| mutL | 5.1 | 81 | 3.0 | 91 | 3.0 | 84 | 1.6 | 84 | 0.9 | 62 | 0.7 | 85 | |
| mutH | 5.1 | 91 | 3.2 | 98 | 2.9 | 85 | 1.5 | 86 | 0.9 | 76 | 0.6 | 79 | |
MF: mutant frequency.
aShown are mutant frequencies obtained at the highest dose of each mutagen tested relative to the frequency for the wild-type strain at the same dose (i.e. wild-type = 1).
bViabilities are shown by the percentages of colony forming units in the presence of mutagen compared with that in the absence of mutagen.