Figure 7.
The 70-kDa FN fragment inhibits HT1080 aggregate formation. HT1080 cells (2.5 × 106 cells/ml) were cultured in hanging drop assay in the presence or absence of 10-7 M dexamethasone (A). Hanging drops were harvested and the assembly of high-molecular-weight FN multimers assessed as described in Figure 3. In separate experiments, HT1080 cells were cultured in FN-depleted medium (B and D) or medium supplemented with 50 μg/ml FN (C and E). The 70-kDa amino-terminal FN fragment (25 μg/ml) was added to inhibit matrix assembly (D and E). Aggregates were viewed using inverted bright field optics.