Figure 5.

Time-lapse images of Rac1 and Cdc42 activity taken using Raichu-CRIB-X. Cos1 cells expressing Raichu-CRIB-X were stimulated with EGF. FRET images were obtained every 30 s for 1 h, and data at the indicated time points are shown as in Figure 1 (n = 5). Note that, in the Raichu-CRIB-X-expressing cells, the FRET efficiency inversely correlated with the local concentration of the endogenous Rac-GTP and Cdc42-GTP. Bars, 10 μm.