Table 2.
Summary of phenotypes of β′- and α-COP mutants
| Alleles | Changes | Defects in vivo/in vitro | References |
|---|---|---|---|
| sec27-1 (β′-COP) | G688D | ts; no KKTN transport defect and no defect for Emp47p (KTKLL) stability at permissive temperature; after ts-shift α-COP instability and both KKTN and KTKLL transport defects | This study; Duden et al., 1994; Letourneur et al., 1994; Schröder-Kohne et al., 1998 |
| sec27-95 (β′-COP) | S114Y | ts; strong defect for Emp47p (KTKLL) stability; no KKTN transport defect | This study; Prinz et al., 2000 |
| sec27Δ1-285 (β′-COP) | residues 1-285 deleted | ts; strong defect for Emp47p (KTKLL) stability; no KKTN transport defect; defective for binding KKTN and KTKLL motifs in vitro | This study |
| ret1-1 (α-COP) | G227D | weak ts; no defect for Emp47p (KTKLL) stability, strong KKTN transport defect; defective for binding to KKTN, but able to bind KTKLL motifs in vitro | Letourneur et al., 1994; Schröder-Kohne et al., 1998 |
| ret1Δ1-285 (α-COP) | residues 1-285 deleted | ts; no defect for Emp47p (KTKLL) stability, strong KKTN transport defect; defective for binding KKTN and KTKLL motifs in vitro | This study; Eugster et al., 2000 |
ts, temperature sensitive.