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Endogenous mouse PLD2 is found on the plasma membrane in multiple types of cell lines and primary cells. Mouse cell lines and dispersed adult cardiomyocytes were fixed and immunostained using monoclonal anti-mouse PLD2 antibody #2 followed by labeled secondary. Confocal images were collected at the midcell level. PLD2 localization to membrane ruffles is observed in the Swiss 3T3 fibroblasts. The dispersed adult cardiomyocytes exhibit a characteristic rectangular morphology.
