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. 2012 Oct 8;591(Pt 1):77–91. doi: 10.1113/jphysiol.2012.243063

Figure 3. Quantification of the effects of donor age and GDNF over-expression on graft size and integration following intranigral transplantation.

Figure 3

A, quantification of GFP+ cells revealed significantly larger grafts from E10 donor tissue compared to E12. GDNF had little effect on the survival of either E10 or E12 donor tissue. P < 0.0001, F= 13.02. B, assessment of the number of graft-derived GFP+ axons at a point midway along the MFB revealed a significant increase in fibre density from younger versus older donor tissue. GDNF significantly increased the number of fibres within the MFB of both young and old donor grafts, with E10+GDNF showing the most robust innervation. P < 0.0001, F= 16.53. C, donor age had little effect on the proportion of cells within intranigral grafts capable of projecting through the MFB (GFP+ fibres within the MFB/GFP+ cells). However, exposure of the grafts to GDNF significantly increased the ability of E10 and E12 grafts to form long distance projections. P < 0.0001, F= 11.17. Open bars: E12 donor graft (n= 21), light grey bars: E10 donor graft (n= 22), dark grey bars: E12 donor graft+GDNF (n= 11), filled bars: E10 donor graft+GDNF (n= 10). Data represent mean + SEM. *P < 0.05, **P < 0.01, ***P < 0.001.