Figure 6. Charge neutralizing mutations in S4 alter the temperature dependence of Drosophila transient receptor potential A1.

A, voltage-dependent activation of H990A-R1004N (n = 5) (A) and H990Q-R1004A (n = 4) (B) at 32°C. (Insets show representative whole cell current traces at 32°C in response to the voltage protocol indicated in Fig. 2D.) C, the H990A-R1004N (n = 4) and (D) H990Q-R1004A (n = 6) mutants have a higher temperature activation threshold.