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. Author manuscript; available in PMC: 2014 Apr 16.
Published in final edited form as: Biochemistry. 2013 Apr 4;52(15):2638–2648. doi: 10.1021/bi3016108

Figure 5.

Figure 5

TonBΔTMD dimerizes in solution. a) Dipolar evolution data obtained by DEER for TonBΔTMD in solution for spin labels at sites 187, 194 and 224. The data for 187 and 224 were recorded at Q-band. The amplitude in the modulation varied between samples, and either reflects different levels of dimerization in the TonBΔTMD preparation or differences in spin-labeling efficiency. The red traces represent the best fit to the data and yield the distance distributions shown in b). The error bars, which demark the shaded region in the distance distribution, indicate the range of solutions that can be obtained given the maximum error in the background subtraction. c) Structure of the intertwined dimer (PDB ID: 1IHR). The two high-resolution structures (PDB ID: 1IHR or 1QXX) provide reasonable matches to the pulse EPR data (Table 2).