Table 1. Loci, primers and annealing temperatures for polymerase chain reactions used in this study.

Locus	Annealing temperature	PCR/sequencing primers	Reference
Autosomal
Recombination activating protein 1 (RAG 1)	65	R50: CTGATCTGGTAACCCCAGTGAAATCC	Irestedt et al., 2001
		R51: GACCCTCTTTCTGCTATGAGGGGGC	Irestedt et al., 2001
		RAG1: CTTCTCACTGACATCTCCCATTCCA	This study
		RAG632F: CAGCCTTGGTTTGTGCCT	This study
		RAG632R: CTCCTCCTCCATGTCTTTTAA	This study
β-fibrinogen gene, intron 5 (FIB 5)	55	Fib5: CGCCATACAGAGTATACTGTGACAT	Fuchs et al., 2004
		Fib6: CCATCCAACATCTCAGCATGATGAAA	Fuchs et al., 2004
Sex-linked
Chromo-helicase-DNA-binding protein, intron a (CHD a)	55	F2550: GTTACTGATTCGTCTACGAGA	Ellegren and Fridolfsson, 1997
		R2718: ATTGAAATGATCCAGTGCTTG	Ellegren and Fridolfsson, 1997
		CHD1F: TATCGTCAGTTTCCTTTTCAGGT	Lee et al., 2010
		CHD1R: CCTTTTATTGATCCATCAAGCCT	Lee et al., 2010
	65	R1: CCATCAAGCCTCTACAAAGATT	This study
		R2: CCATCAAGCCTCTACAAAGATA	This study
Brahma protein, intron 15 (BRM 15)	55	F: AGCACCTTTGAACAGTGGTT	Borge et al., 2005
		R: TACTTTATGGAGACGACGGA	Borge et al., 2005
Very low density lipoprotein receptor, intron 9 (VLDLR 9)	55	F: AAGTGTGAATGTAGCCGTGG	Borge et al., 2005
		R: TCGGTTGGTGAAAATCAGAC	Borge et al., 2005