Figure 10.

Analysis of the effect of phagocytosis of apoptotic or necrotic cells on the expression of biologically active IL-6 and TNF. Mf4/4 cells were coincubated for the indicated time with untreated or FasL-treated apoptotic L929hFas cells, or with untreated or R32WS86T-treated necrotic L929hR55 cells. Incubation medium was collected 24 h later and the concentrations of biologically active IL-6 and TNF were determined. Positive controls of Mf4/4 cells treated for 2 and 24 h with LPS and negative controls of untreated and FasL- or R32WS86T-treated target, and Mf4/4 cells are also presented. Measurements are presented in logarithmic scale. Error bars represent the SD of at least three independent experiments.