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. 2013 Feb 28;288(16):11520–11530. doi: 10.1074/jbc.M113.460972

FIGURE 3.

FIGURE 3.

Subcellular distribution of candidate CPE synthases. A, confocal sections of Drosophila S2 cells double-transfected with V5-tagged dCCS1, dCCS2, dCCS3, or dCCS4 and ER marker PE-methyltransferase-GFP. Cells were stained with anti-V5 antibody (top) or co-stained against the V5 epitope and Golgi marker dGMAP (bottom). Arrows indicate nuclear envelope staining, whereas arrowheads mark immunopositive Golgi structures. B, confocal sections of human HeLa cells transfected with V5-tagged dCCS1, dCCS2, dCCS3, or dCCS4 and stained with anti-V5 and anti-calnexin (ER) or anti-GM30 (Golgi) antibodies. Scale bars: 5 μm.