TABLE 5.
Methylation linkage analysis of the WIC29.26 epitope
| PMAA derivative | Residue type | Relative TIC areaa | Retention time |
|---|---|---|---|
| min | |||
| 1-[2H]2,3,4-Tri-O-methyl-1,5-di-O-acetyl-rhamnitol | t-Rhap | 0.1 | 17.00 |
| 1-[2H]2,3,4-Tri-O-methyl-1,5-di-O-acetyl-fucitol | t-Fucp | 0.1b | 18.24 |
| 1-[2H]2,3,4-Tri-O-methyl-1,5-di-O-acetyl-xylitol | t-Xylp | 0.6 | 18.55 |
| 1-[2H]2,3-Di-O-methyl-1,4,5-tri-O-acetyl-rhamnitol | 4-Rhap | 0.4 | 20.54 |
| 1-[2H]2,3,4,6-Tetra-O-methyl-1,5-di-O-acetyl-galacitol | t-Galf | 0.7 | 21.33 |
| 1-[2H]3,4-Di-O-methyl-1,2,5-tri-O-acetyl-fucitol | 2-Fucp | 0.8 | 21.49 |
| 1-[2H]2,3,4,6-Tetra-O-methyl-1,5-di-O-acetyl-galacitol | t-Galp | 2.6 | 21.70 |
| 1-[2H]2,3-Di-O-methyl-1,4,5-tri-O-acetyl-xylitol | 4-Xylp | 1.0 | 22.08 |
| 1-[2H]2-O-Methyl-1,3,4,5-tetra-O-acetyl-fucitol | 3,4-Fucp | 0.1b | 23.11 |
| 1-[2H]3,4,6-Tri-O-methyl-1,2,5-tri-O-acetyl-galacitol | 2-Galp | 1.1 | 24.59 |
| 1-[2H]1,2,3,4,5-Penta-O-acetyl-fucitol | 2,3,4-Fucp | 1.1 | 25.03 |
| 1-[2H]1,2,3,4,5-Penta-O-acetyl-xylitol | 2,3,4-Xylp | 0.4 | 27.27 |
| 1,5,6-Tri-O-methyl-3,4-di-O-acetyl-2-N-methyl-acetyl-1-[2H]glucitol | 3,4-GlcNAc-OL | 1.0 | 28.96 |
a Relative total ion current (TIC) relative to that for 4-O-Xylp = 1.0.
b Residues suggesting heterogeneity in the substitution of Fucp residues C and J by Rhap residues B and L, respectively.