FIGURE 1.

ZEB1 is induced with Rb1 family mutation in MEFs, and Rb1 family mutation leads to loss of cell contact inhibition. A, real-time PCR comparing ZEB1 mRNA expression in wild-type MEFs with MEFs with the indicated Rb1 family mutation. TKO indicates MEFs with all three Rb1 family members mutated (6). Actb is β-actin, included as a control. Error bars, S.D. B, subconfluent TKO MEFs. C, TKO MEFs at confluence. The arrow denotes initial foci formation resulting from loss of cell contact inhibition. D, TKO MEFs after 2 weeks at confluence. The arrow denotes initial foci that have progressed to form a large mound of cells.