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NAADP levels increase transiently in response to activation of muscarinic receptors. Shown are NAADP levels in guinea pig tracheal rings over time after exposure to the muscarinic agonist carbachol (100 μm). Error bars represent the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01. Means were compared by analysis of variance (p = 0.03), followed by Dunnett's multiple comparisons test against time 0.
