FIGURE 2.

Activity assays of MtuMutT1, EcoMutT, and Rv1700 proteins on 8-oxo-dGTP/8-oxo-dGDP (A) and 8-oxo-GTP (B). Approximately 250 μm 8-oxo-G nucleotides were incubated with ∼100 ng of MtuMutT1, Rv1700, and EcoMutT, as indicated in a 10-μl reaction in 1×MutT buffer for 30 min at 37 °C. The reactions were stopped with 0.1% SDS and analyzed by HPLC using a DNAPac column with buffer A consisting of 25 mm Tris-HCl, pH 9.0, and 1 m lithium chloride as buffer B. Lithium chloride gradient (0–40%) was set at a flow rate of 0.5 ml/min for 25 min. Various panels are as indicated.