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. 2013 Mar 5;288(16):11252–11262. doi: 10.1074/jbc.M112.442566

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Mass spectrometric analysis of the 8-oxo-G nucleotides and the reaction products with MtuMutT1 and/or Rv1700 using MALDI-MS. Panels: i, 8-oxo-dGTP standard; ii, 8-oxo-dGDP standard; iii, 8-oxo-dGMP standard; iv, 8-oxo-dGTP treated with MtuMutT1; v, 8-oxo-dGTP treated with EcoMutT; vi, 8-oxo-dGTP treated with MtuMutT1 and Rv1700; vii, 8-oxo-dGDP treated with Rv1700; viii, 8-oxo-dGTP treated with Rv1700; ix, 8-oxo-GTP standard; and x, 8-oxo-GTP treated with MtuMutT1. The m/z values of the various peaks are as shown. Theoretical masses for singly charged peaks of 8-oxo-dGTP, 8-oxo-dGDP, 8-oxo-dGMP, 8-oxo-GTP, 8-oxo-GDP, and 8-oxo-GMP are 523.99, 444.02, 364.06, 539.99, 460.02, and 380.05 Da.

FIGURE 3. — Mass spectrometric analysis of the 8-oxo-G nucleotides and the reaction products with MtuMutT1 and/or Rv1700 using MALDI-MS. Panels: i, 8-oxo-dGTP standard; ii, 8-oxo-dGDP standard; iii, 8-oxo-dGMP standard; iv, 8-oxo-dGTP treated with MtuMutT1; v, 8-oxo-dGTP treated with EcoMutT; vi, 8-oxo-dGTP treated with MtuMutT1 and Rv1700; vii, 8-oxo-dGDP treated with Rv1700; viii, 8-oxo-dGTP treated with Rv1700; ix, 8-oxo-GTP standard; and x, 8-oxo-GTP treated with MtuMutT1. The m/z values of the various peaks are as shown. Theoretical masses for singly charged peaks of 8-oxo-dGTP, 8-oxo-dGDP, 8-oxo-dGMP, 8-oxo-GTP, 8-oxo-GDP, and 8-oxo-GMP are 523.99, 444.02, 364.06, 539.99, 460.02, and 380.05 Da.