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miR-155 negatively regulates iNOS expression in MSCs. MSCs were transfected with 80 nm negative control (NC) or miR-155 mimics (A–C) or inhibitors (D–F). After 24 h, medium was discarded, and fresh medium either lacking (control) or containing 10 ng/ml IFNγ and 10 ng/ml TNFα was added. The level of nitrate in the supernatant (B and E), iNOS mRNA (A and D), and protein were determined 24 h later by Griess test, real-time PCR, and Western blotting analysis, respectively.
