FIGURE 10.

Effect of Casodex on ELK1-dependent gene activation by androgen/AR. A, LNCaP cells plated in hormone-depleted medium were treated with vehicle (Veh) or R1881 (1 nm) or R1881 (1 nm) + Casodex (10 μm). 48 h after treatment, cells were harvested to measure mRNA levels of the indicated genes by quantitative real time PCR. A, inset, Western blot for AR expression following the different treatments with GAPDH as the loading control. B, C4-2 cells plated in hormone-depleted medium were treated with vehicle or R1881 (1 nm) or R1881 (1 nm) + Casodex (10 μm). 48 h after treatment, cells were harvested to measure mRNA levels of the indicated genes by quantitative real time PCR. B, inset, Western blot for AR expression following the different treatments with GAPDH as the loading control. * and §, p < 0.001. Error bars represent S.D.